| Aseptic Technique/Gram Stain | Archaea | Bacterial Friends & Foes | Gram Positive Pathogenic Bacteria | Gram Negative Pathogenic Bacteria |
|---|---|---|---|---|
|
30 seconds
How long do you keep crystal violet stain on your slide?
|
1977
When were archaea bacteria discovered?
|
Promote gut health and normal flora, and take up
space so that pathogenic bacteria can not grow.
Name two ways that probiotics are beneficial to
humans? |
Streptococcus pyogenes
Gram + beta-hemolytic coccus known to cause strep throat and scarlet fever
|
Neisseria, Chlamydia, Treponema
Which of the following genera are associated with sexually transmitted diseases?
|
|
100x
When using oil immersion, what objective do you use the oil?
|
Peptidoglycan
What don't archaea cell walls have?
|
Penicillin (antibiotic), Streptomyces (antibiotic),
Rapamycin (immunosuppressant), Bleomycin (chemo drug).
Name two medicines that bacteria make which are
beneficial to humans. |
Micrococcus
Gram + bacteria genus characterized by cocci with exceptionally thick cell walls that stain intensely purple
|
Salmonella and Shigella
Gram - bacillus; non-lactose fermenter, the common cause of food poisoning
|
|
To decolorize the crystal violet
Why do we use alcohol when gram staining slides?
|
One (monolayer)
How many layers do archaea cell walls have?
|
In the Food and Pharmaceutical Industries
In what two industries are bacteria used as a source of
enzymes? |
Staphylococcus aureus
Gram + coccus that is a common cause of nosocomial infection and has developed extensive antibiotic resistance
|
Bordetella pertussis
Gram - coccobacillus; causative agent of whooping cough
|
|
The medium in the tube might become contaminated by microbes in the air.
If you forget to flame the mouth of a broth culture tube before transferring culture to it, what might result?
|
No
Are archaea known to cause disease in humans?
|
Lactic Acid Bacteria
What bacteria are fermentative bacteria, help with
producing certain foods, and obtain their energy from metabolized sugars? |
Bacillus
Gram + genus characterized by large spore-forming rods that are facultative anaerobes and are commonly found in soil
|
Helicobacter pylori
Gram-helical; multiple flagella; halophilic; causative agent of stomach ulcers
|
|
3, 1, 4, 2
Place the steps of the Gram Stain in order by number:
1) Flood slide with Gram's Iodine for 1 minute; wash with water 2) Flood slide with Safranin for 30 seconds; wash with water 3) Flood slide with Crystal Violet for 1 minute; wash with water 4) Decolorize slide with alcohol for 10-20 seconds; wash with water |
Archaea cells are structurally different from bacterial cells, so they aren't targeted by antibiotics.
Why wouldn't antibiotics work on archaea?
|
Make antibiotics and medicine, found in our normal
flora, ingest them to promote a healthier gut, can be used as insecticides, and take up space so that pathogenic bacteria can not grow.
Name different ways that bacteria can be/ are
beneficial to humans (at least 4). |
Corynebacteria diphtheria
Gram + club-shaped rod that produces a powerful toxin that causes inflammation of the mucous membrane: babies are vaccinated in developed countries
|
Mycobacterium
Gram variable bacillus; obligate aerobe; surrounded by waxy envelope giving it resistance; stained with acid-fast stain; causative agent of leprosy and tuberculosis
|
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