All About Proteins | Equipment | Fingerprinting | Western Blotting | Structure |
---|---|---|---|---|
What are amino acids?
The monomers (building blocks) of a protein.
|
What is a micropipette?
The device used for measuring, drawing up, and dispensing small amounts of liquid.
|
What is translation?
The step where genetic code carried by mRNA is decided to produce the specific sequence if amino acids in a polypeptide chain
|
What is a protein?
Detects specific _____ in a tissue sample
|
What is the secondary structure?
The structure in which the amino acids form an alpha helix
|
What is a protein?
One of the four macromolecules of biology, this molecule does everything in a cell except hold/transport genetic information and energy.
|
What is a power source?
The device in which the electrodes are plugged in and receive power.
|
what is transcription?
The step where info in a strand of DANA is copied into a new molecule of mRNA
|
What is a polyacrylamide gel?
The type of gel used in Western Blotting
|
What is the primary structure?
The structure in which the amino acids form a single file line
|
What is (vertical) gel electrophoresis?
The process by which proteins can be separated based on size and weight.
|
What is the chamber?
The contraption that holds the gels.
|
What is DANA?
deoxyribonucleic acid
|
What is a membrane?
The proteins are transferred on to a ________
|
What are amino acids?
The chemicals which come together to form proteins
|
What is the central dogma of biology?
The process by which mRNA take information from DNA, transport out of the nucleus, and go to a ribosome, where amino acids are linked onto tRNA.
|
What is a heat block?
The machine which heats up samples (without water).
|
What is mRNA?
Messenger ribonucleic acid
|
What is immunoblot?
Another name for Western Blotting
|
What is tertiary structure?
The structure in which the protein blobs upon itself
|
What is Biuret's reagent?
The common indicator that tests for a protein.
|
What is a Western Blotting cassette?
The contraption that holds the gel, a nitrocellulose membrane, and pads.
|
tRNA
Transfer ribonucleic acid
|
Southern, Northern, Western
The other types of blotting
|
What is quaternary structure?
The structure in which multiple protein blobs come together
|