Freezing Concentration

This Reagent forces Platelets to form large Aggregates that are heavier than Lymphocytes.

Frozen samples in small cryovials are transferred to what? This is so they can be diluted sufficiently to minimize toxicity.

Lymphocytes and Monocytes have a density of

When doing a Manual Freezing, it is important to leave the cells in the -20 C Freezer for a minimum of

Tissues we can use to isolate Lymphocytes

Lyses RBC

This needs to be removed because it is covered with HLA class I receptors and will bind to HLA Antibody which will decrease Antibody available for Lymphocytes

The Liquid Nitrogen tank should be between these Temps

If the cell to DMSO Ratio is not correct this could lead to

5x10^6

A Denstiy Gradient that has a specific gravity that is the sameas Lymphocytes

This Reagent is recommended to use when cleaning up frozen Lymphocyte preps before running FCXM because this removes any Non-Viable Cells

This is One of the main reasons we freeze Donor Cells

If the cell prep is not cleaned properly, it can give results that are

This helps the Cells stay "Happy"

This is a Cryopreservative that is essential to prevent cell damage due to ice crystal formation

Cell Viability should always be greater than

This is when we check the Nitrogen Tank Levels!!! Lol

DMSO is toxic to lymphocytes at room temperature, so cell should be

Our Cells are frozen in what type of tubes

By ingestion this Reagent, it aids in the removal of Monocytes by passing them over a magnet

When counting your Lymphocytes for freezing on the Hemocytometer, We do not include cells that are on the

We freeze how many Tubes per Organ

A scatter plot with Dual Populations or with very FEW T or B Cells gives DR. K