Gem 2018 Lab Jepardy Jeopardy Template

PCR	Microscopy	General practice	Papers	Final Jepardy
100 Denaturation 94-95, Annealing 50-54, Extension/elongation 72 What are the Cycles of PCR with Temperatures	100 What is 100X. The objective power for counting cells with oil immersion.	100 10% HCl To prevent growth, bottles are stored with what liquid at what concentration?	200 Observable difference between cultured microbes and those from a pure water sample seen under a microscope. Which method represents actual species living in the wild. Briefly describe "The Great Plate Count Anomaly"	500 You need to send these sequences out for sequencing which requires all samples to be 20uL at a 20ng/uL concentration, or at least 500ng of sample DNA. Do the dilution calculations and fill in the Labels.
200 Binding, wash, elute. What are the Steps involved in spin column based PCR cleanup.	200 What is SYBR green works by binding DNA molecules The stain used in lab for cell counting and how it works.	200 Pipette gun = 5mL P1000 = 350 How would you PRECISELY pipette 5350uL in less than 3 draws?	200 Giavannoni et. al. 1990 Who solved the great plate count anomaly citing the plethora of unculturable SAR11 sequences?
300 Less specificity of primer binding due to low annealing temperature. Increase the annealing temperature What is the cause and solution of a primer dimer	300 What is increases resolution by decreasing the angle of refraction. The purpose of immersing the objective in oil.	100 20 - 200 uL What is the range of our P-200s?	100 Nitrogen What major nutrient is considered the most involved in the growth of phytoplankton?
100 200 - 1000 uL What is the range of our P-1000s?
100 .5 - 10 uL What is the range of our P-10s?

GEM 2018 - Lab Jepardy

Press F11 for full screen mode