PCR	Gel electrophoresis
100 a specific area of DNA What is the template in PCR?	100 Pieces of DNA of different sizes What are the bands on the gel?
200 primers What is added to the PCR reaction that determines the portion of DNA amplified in a PCR reaction?	200 Multiple alleles, non specific binding What is the reason for multiple bands when amplifying the same area of DNA?
300 Taq polymerase, MgCL2, dNTPS, buffer, What are the other components of a PCR reaction beside template and primers? (4 items)	300 Heterozygote What is the genotype of someone whose PCR of Alu resulted in 2 DNA bands?
400 Denaturation: 94 Annealing: 55 to 62 Extension: 72 What are the temperatures at which denaturation, annealing, and extension take place?	400 mutation or inheritance of different copies of a gene What is the cause of different alleles?
500 denaturation: heat separates DNA annealing: primers bind extension: Polymerase adds dNTPS to synthesize a copy of fragment What are the 3 stages and what occurs during each stage?	500 An electrical current is applied to the buffer which generates a positive and negative pole. The DNA is negatively charged and is drown toward the positive pole. What causes the DNA to move through the gel in gel electrophoresis? (Be sure to mention negative charge, positive charge, buffer, and electrical current.)

Final Review 2

How many teams are playing ?
Time given to answer each question ?