| Pax Genes | Genetic Building Blocks |
|---|---|
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Pax-A, Pax-B, Pax-E
What are the three Pax genes discussed in the paper?
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Photoreceptors convert light into intracellular signals. The two types of PRCS mentioned are ciliary and rhabdomeric.
What is the purpose of photoreceptors? What are the two types of PRCs mentioned in this paper?
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Pax-A was expressed in the eyes, Pax-B and Pax-E were expressed in the manubrium, feeding and reproductive organs
Where were the Pax genes expressed in Cladonema?
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The EST of the Tripedalia opsin was most similar to that of c-opsin
How did researchers discover that the Tripedalia opsin was c-opsin?
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Caused ectopic eye formation
What effect did Pax-A have on Drosophila?
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1. Oca-2
2. Mit-f
What were the two things researchers tested for when identifying the pigment?
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ML is a statistical method of phylogenetics, which calculates the probability of a particular character changing at any point on a phylogenetic tree.
Describe the Maximum Likelihood (ML) method.
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J1-crystallin is the major protein of the lens in the Tripedalia eye. They tested for its presence using immunohistochemistry and it was expressed in the slit and pit eyes, as well as the lens.
What is J1-crystallin? How did they test for its presence? If present, where was it expressed?
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Pax-A is expressed in Cladonema eyes and induced eye formation in Drosophila; the Pax duplications occurred before cnidarian-bilaterian split. That is, animal eyes are monophyletic.
What were the results? What was the conclusion?
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Tripedalia has:
1. Ciliary PRCs 2. c-opsin (found through EST) 3. Melanin (Oca-2, Mit-f) 4. They concluded that the camera-type eye and vertebrate eye have many genetic similarities - arose through independent recruitment.
Sum it all up! (PRCs, opsins, pigment, conclusion)
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