Dna Sequencing Sanger Method Jeopardy Template

History	Dideoxynucleotides	The process	Applications	Limitations
100 Fred Sanger Who discovered the Sanger Method?	100 Nucleotides that lack a hydroxyl group, chain-terminating nucleotide What are dideoxynucleotides?	100 Step that takes place prior to the denaturing and priming of the DNA What is DNA purification?	100 Agriculture, medicine, forensics What were the mentioned fields of science?	100 method that is better for larger projects, and is the more commonly used method What is Next-generation sequencing?
200 1977 What year was the Sanger method discovered in?	200 Nitrogenous base What is a dye molecule on a dideoxynucleotide linked to?	200 primer is added What happens after the double helix separates into single strands?	200 Used to create genetically modified organisms How does DNA sequencing apply to agriculture?	200 too expensive What is the Sanger method not good for large projects?
300 Sanger method What was one of the earliest methods for DNA sequencing?	300 3' carbon of the sugar ring Where is the hydroxyl group on a normal nucleotide?	300 An enzyme that catalyzes the synthesis of DNA What is DNA polymerase?	300 To match suspect DNA to the DNA found at a crime scene How does DNA sequencing apply to forensics?
400 A dideoxynucleotide What does the chain end with?	400 DNA polymerase + free nucleotides What is found in each test tube?
500 DNA polymerase, DNA primer, nucleotides, template DNA, dideoxynucleotides What are the 5 main ingredients required for Sanger sequencing?	500 this process is used to sequence the DNA What is Gel Electrophoresis?

DNA SEQUENCING -- SANGER METHOD

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