Phytoremediation | Bioindicators and Biomarkers | Organic Contaminants | Metals and Radioactive Isotopes | Toxicity Test Methods |
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What is Plant Growth Promoting Rhizobacteria (PGPR)?
Plants are coated with this bacteria, it promotes growth by decreasing ethylene stress and producing auxins.
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What is a bioindicator
Biological responses to environmental chemicals at higher organizational levels. Eg. population, community, ecosystem
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What are modifying factors in mechanisms of toxicity.
Mixtures, sunlight, temperature, drought, pests, partitioning.
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What is somatic radiation damage
This type of radiation damage affects proteins more than membranes, it alters the secondary and tertiary structures of them, altering their function. Classified as acute cytotoxicity.
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What is vibrio fischeri
This commonly used bacterial assay uses the luminescence of this microbe as the indicator. A 30 min assay.
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What is petroleum hydrocarbon?
60% of contaminated sites in Canada are contaminated with this toxicant.
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What is the aminolevulinic acid dehydratase (ALAD) assay
A key step in heme biosynthesis, this biomarker is very reliable and measures the impact of lead on the organism.
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What is glyphosate (Roundup)
This herbicide inhibits metabolism, inhibits the Shikamate pathway, rapidly degrades in soil and is non-toxic to non-plant species
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What are metallothioneins.
It offers protection against ROS toxicity due to the high Cys content. Its expression is induced by metals and ROS, and can remove toxic metal from other macromolecules in the cell.
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What is lemna gibba
This plant assay uses the inhibition of growth based on frond production as the indicator. An 8 day assay
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What is microbial aerobic PHC degradation?
PHC is degraded through this process by hydroxylating it into fatty acid to be used as food.
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What is the ethoxy-resorufin-o-deethylase (EROD) assay
An assay used where the biomarker is the transformation of ethoxy-resorufin to hydroxy-resorufin which increases the fluorescence.
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What is paraquat.
A free radical generator, it does it to both chloroplasts and mitochondria, it accepts electrons from photosystem I and passes them to oxygen to from superoxide.
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What is the biological ligand model (BLM)
This process directly binds onto biological receptors such as membranes, ion channels, enzymes, etc. It generally dominates at higher concentrations and is more likely to have acute effects.
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What is daphnia magna
The most commonly used test, it uses the death of the organism as the end point and is a 48hr acute toxicity test.
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What is phytoextraction?
Toxicants are taken up into the leaves, the leaves are harvested, and the contaminant extracted from them.
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What is the photosynthesis assay
An assay using the Calvin cycle or electron transport chain as a biomarker. Measures impact and detects many classes of toxicants.
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What are organochlorines
DDT is an example of this pesticide, which retards closure of sodium channels to cause immobilization of target species. It's a carcinogen and endocrine disruptor. Contains chlorinated ethanes.
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What is reactive oxygen catalytic mechanism (RCM)
It is the catalytic production of ROS species with amplification. It generally dominates at lower concentrations and more likely to have chronic effects.
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What is hyallela azteca
This test is used for sediment assays. Growth & survival, or reproduction assays are options. 1-4 week assay.
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What is physical soil treatment?
Tilling the soil, exposure to sunlight and air which photooxidizes contaminants.
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What is the gene expression assay
Micro-array techniques, ddPCR and identification of differentially expressed cDNAs are techniques used for this assay.
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What are organophosphates
This pesticide inhibits acetylcholine esterase, is more degradable and less persistent than organochlorines yet more acutely toxic to non-target species.
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What is copper
This metal is able to redox cycle in the RCM thus highly toxic in high concentrations. It is more toxic than zinc and it binds to DNA between bases disturbing the base pairing (BLM)
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What is avian toxicity testing
This test uses the production of fertile viable eggs as the endpoint, it is required by the FIFRA and Toxic Substances Control Act. Acute oral toxicity or sub-acute toxicity are other assays used
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