Bacterial Enumeration and Reproduction | Bacterial Cell Structure and Cell Wall Synthesis | Aerobic Respiration/Methods of Energy Production | Microscopy and Important Figures in MBio | Endospores and Random Bacteria Stuff |
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What is the formula for final bacterial growth and number of generations?
N=No2^n and n=3.3(logN-logNo)
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What is the difference between pili and flagella and what component of bacterial cells controls the movement of flagella?
Pili - Attachment
Flagella - Motility; controlled by chemotaxis |
What are the final products of Glycolysis?
2 Pyruvate, 2 NADH, and 2 ATP (4 total ATP but 2 are used for the first phase of glycolysis (Glucose to G3P))
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What is Koch's Postulates?
1.) The bacteria must be present in every case of the disease.
2.) The bacteria must be isolated from the host with the disease and grown in pure culture. 3.) The specific disease must be reproduced when a pure culture of the bacteria is inoculated into a healthy susceptible host. 4.) The bacteria must be recoverable from the experimentally infected host. |
What is an endospore and which type of bacteria utilizes one?
An endospore is an "escape pod" for the genetic material of a gram + bacterium in which a compartment for the DNA will be made when the mother cell is in distress and could possibly die due to being in an environment lacking in essential needs like nutrients or correct temperature/pH.
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What is binary fission and what are the steps?
A form of asexual reproduction seen in bacteria, and the steps are : 1.) Replication Stage 2.) Growth Stage 3.) Segregation Stage 4.) Splitting
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What is the last and arguably most important step of peptidoglycan synthesis for the cell wall and which antibiotic inhibits it?
Transpeptidation; Penicillin
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What is the Kreb's/TCA Cycle for and what are the final products?
The production and distribution of electrons for electron carriers to power the ETS/ETC, and the final products are 6 NADH and 2 FADH2
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What is total magnification and how does magnification work?
The product of the ocular magnification and the objective magnification; through the refractive indexes of curved glass.
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What are all the types of bacterial cell morphology?
1.) Bacillus (strepto- and diplo-)
2.) Spirochete (vibrio or comma-shaped, spirillum or a thick and rigid spiral, and spirochete or just a flexible spiral) 3.) Coccus ( strepto-, staphylo-, diplo-, tetrad and sarcina) |
What is the difference between selective and differential media?
Selective: Includes nutrients/components needed for specific microbial growth, Differential: Includes a dye indicator to showcase presence of specific microbial growth
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What is the main structural difference between eukaryotic cells and prokaryotic cells that we discussed in class?
No true nucleus in bacteria (prokaryotic cell) vs a true nucleus in eukaryotic cells, so genetic material in region called nucleoid. This means it is without a nuclear membrane.
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What is the ETS/ETC and how does it generate a PMF? (electron transport chain/system and proton motive force)
It is the energy force needed to power the production of ATP by powering ATP synthase to create a hydrogen ion gradient across the membrane of the bacterium. This is done by the lowering of energy states of electrons as they are picked up by a terminal electron acceptor (O2 in the case of aerobic respiration).
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What are Florence Nightingale and Louis Pasteur known for in microbiology?
Florence Nightingale developed proper methods of sanitation during war after noticing the amount of preventable deaths due to microbes, and Louis Pasteur is known for disproving spontaneous generation of microbes and pasteurization of dairy products.
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What are the steps of endospore formation?
1.) septum forms - DNA replicates
2.) septum separates forespore from mother cell - DNA pumped into forespore 3.) mother cell engulfs forespore 4.) forespore develops cortex of peptidoglycan 5.) mother cell breaks down, endospore released |
What is the main difference between a conjugation pilus and regular pili?
A conjugation pilus is used for DNA transfer between prokaryotic organisms (bacteria) and regular pili are used for cell to cell attachment.
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From outside to inside, (capsule to cytoplasm), list all the structures and membranes found in a bacterium, and list at least 3 structures found within the cytoplasm.
Capsule, envelope, cell wall, cell membrane, cytoplasm
Found in cytoplasm: ribosomes, DNA/RNA, endospore (gram +), transcription and translation material like DNA polymerase, RNA polymerase, etc, proteins, cytoskeletal structures. |
What are the reduced and oxidized states of the two main electron carriers seen in aerobic respiration.
NADH = reduced
NAD+ = oxidized FADH2 = reduced FADH = oxidized FAD = further oxidized |
What are Antony Van Leeuwenhoek and Sir Robert Hooke known for in microbiology?
Leeuwenhoek developed the very first microscope, and Sir Robert Hooke improved the method of microscopy by inventing the compound microscope
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What are the phases of bacterial growth and what is happening at each one?
1.) Lag phase - preparing cell replication machinery
2.) Log phase - exponential cell reproduction/growth 3.) Stationary phase - maintaining steady reproduction, numbers of bacteria become stagnate. 4.) Death phase - bacteria start to die |
What is the "Plate Anomaly" and why does it exist?
Only ~0.1 of microbes found in nature can grow in manipulated (lab) environments. This is due to nature having way more access to raw materials and nutritive components, as compared to a lab setting.
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What is the peptidoglycan layer and what are its components, where is it found, and what is the difference between it in gram + and gram - bacteria?
Protective/Structural component of the bacterial cell located in the cell wall, composed of sugar groups (NAG and NAM) and proteins (peptides) connected by peptide bonds. Gram + have multiple layers of peptidoglycan connected by teichoic acids, and gram - bacteria have a thin layer of peptidoglycan with an outer layer of lipopolysaccharides.
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What are all of the sets and subsets of carbon and energy production seen among living organisms?
Energy Source: Light; Autotroph - Phototroph
Energy Source: Chemicals; Heterotroph- Chemotroph- Chemoorganotroph and Chemolithotroph |
What are all the methods of microscopy discussed in class and what are the main differences between them?
1.) Light microscopy (light contrast)
2.) Dark-field microscopy light comes from sides; dark contrast) 3.) Phase-Contrast microscopy (phase ring amplifies differences in refractive index of structures in bacterium) 4.) Fluorescence microscopy (via fluorescence or colored light emitted when hit with a different colored light) 5.) Electron Microscopy (scanning=via electron beam and transmission=electromagnets as lenses) |
What are all of the methods of solute transportation across the cell membrane and what is the difference between primary and secondary active transport?
Passive transport (w/ conc. gradient) and subset facilitated diffusion (facilitated by membrane protein)
Active transport (against conc. gradient) Primary: ABC Transporter, primary= with ATP Secondary: Coupled Transporter, secondary= with concentration gradient of another solute (includes symports and anti-ports) |