Bio 200 Final Part One Jeopardy Template

Rb pathway	Gel electrophoresis	PCR	Neuralation (embryo development) pt. 1	Neuralation (embryo development) pt. 2
100 What is e2f A common protoncogene	100 What is farther toward the positive end of the plate Smaller fragments move	100 What is dénaturation, 95 degrees, hydrogen bonds break, and dna is split into 2 strands (unzipped) Step one of PCR State name, temp, bonds made/broken, and what happens	100 What is Ectoderm, mesoderm, endoderm The 3 parts of the early embryo	100 What is The neural tube Made of Ectodermal cells - will become the central nervous system
200 What is Rb Natuaral inhibitor of e2f	200 What is DNA ladder helps us count number of base pairs	200 What is Anealing, very cold (55), primers are bonded to DNA where we want to amplify, Hydrogen bonds are formed Step 2 of PCR State name, temp, bonds made/broken, and what happens	200 What is endoderm goes on to be cells of the intestine lining and airway lining	200 What is Notochord made of mesodermal cells that release noggin
300 What is adding phosphates way to turn off Rb	300 What is Negative DNA has this charge	300 What is synthesis (intermediate temp) Tach pol uses nucleotides to build new dna, H bonds form, dntps are used by enzymes, and phosphodiester bonds form Step 3 of PCR State name, temp, bonds made/broken, and what happens	300 What is Neural default state of the ectoderm	300 What is neural noggin is present cell will become
400 What is Genes always being expressed and moving from G1 to S when they are not supposed to mutant Rb causes	400 What is Wells Holes in agar plate	400 What is DNA, nucleotides, tach pol, primers, (maybe dntps unclear notes on this) Starting material for PCR	400 What is noggin Helps change from default to something else	400 What is Skin BMP4 present cell will become
500 Binds to a promoter on a sequence of dna and recruits rna pol and the gene immediately following that promoter is transcribed how does e2f work	500 What is phosphodiester bonds Bonds that NEVER break in PCR	500 What is Noggin highly concentrated close to the notochord	500 What is Pluripotent What is the cell fate at gastrolation

BIO 200 final (part one)

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