ID Features Feeding Group & Tolerance RCA OBBN Sampling
100
Sow Bugs
Mini armodillo/potato bugs
uropods bifid
7 pairs of legs
1st antenna longer than the seonc
Isopoda
100
predator w mod tol
tabanidae
100
"Reference concentration approach
Compares benthic commnunities at test sites to multiple minimally impacted reference sites
Combo of catchment and site level habinat measures to select appropriate ref site"
What is the RCA and what does it stand for?
100
"measure natural features that are known to influence biological communities
distinguish ref site groups
useful for building models"
Niche Variables
100
"Option for wadable, sparsely vegetated soft sediment
Steams: no
Lakes: no
Wetlands: yes"
Jab and sweep
200
Scuds
2 long antenna of equal length
7 pairs of walking legs
no colour when preserved
Amphipoda
200
Shredder w mod tol
nematoda
200
"Experimental controls
Minimally impacted by human disturbance
Impacted sites can be used if they are minimally impacted to stressor of interest"
What makes a good reference site and why
200
"3 replicate samples
understanding variability is umportant for bioassesssment (within group vs between group variance)
variance needed for confidence in sampling being representative"
Replication
200
"Any plant diversity
Stovepipe corer
core"
"Wetland
Soft organic Soil
0.05 - 1m deep water"
300
Beetles
3 pairs of jointed thoracic legs
Adults look like terrestrial beetles. Antenna w 11 or fewer segments
Elytra wings that cover body
Coleoptera
300
predator w high tol
hirudinea
300
"Aquatic habitat disruption
point source contamination
riparian veg &deforistation of site and catchment
Dev/Urban/Ag landuse in catchment/site
imperviousness/artifical drainage in catchment
acidification (pH)
Water chem (concentration of disolved ox)
Regulation of water level (dams)"
Considerations when screening potential ref sites
300
"Spread test sites across study area
locate area near imporant nodes (features)
stratify sampling by landuse/type of water body"
Biomonitoring study design - questions about unfamiliar areas
300
"Pros:
High richness
animals large and easily ID'd
sig spring data exists
Cons:
Short sampling period before peak times for spring emergence"
Spring
400
Fishflies/Alderflies
Lateral gill filaments
well developed mandibles
anal prolegs or long terminal filament"
Megaloptera
400
Collector w low tol
ephemeroptera
400
"1 select ref site (minimally impacted & ideally randomly
2. biological conditino is summarized. ref sites grouped by biological community
3. Niche variables identified
4. NV used to build predictive model
5. Biological, habitat, physiographic data collected for test site
6. Test site matched w ref site
7. stats to compare
6 steps for RCA
400
"1. define site boundries
2. Map surface water and identify features of interest
3. Consult local agencies for available monitoring info
4. Determine sampling effort
5. For general questions about unfamiliar areas, spread test sites across study area, locate areas near important nodes, stratify sampling by water body or land use
6. Locate test sites across anticipated gradient from stressor impact
7. Select and samlpe ref sitese
8. Sample all manditory sites"
Biomonitoring study design - 8 steps
400
"Preferred: Marchant
Gives more reliable estimates of sample abundance
easily randomized
costly and time consuming
Optional: Bucket
Fast
Minimal Equipmenet
Inadequate randomization may cause bias"
"Picking sub-sample:
Marchant or Bucket?"
500
Midges
segmented
anterior and posterior parapods w hooks
well developed sclerotized head w eyes"
chrionomidae
500
Collector w low tol
Lepidoptera
500
"Biological data can be compared at test and ref sites
can be used to:
assess impact
diagnose stressors
evaluate temporal and spatial trends
provide data for water management"
What does the RCA assume?
500
"1. summarize data using set of metrics
2. Predict a test site to a set of ref sites using NV
3. Use ref site to establish what is normal
4. Test hypothesis"
"Assessing biological condition
4 step process"
500
"Preferred: PresrevedREquired if samples are stored
alcohol or formulin
removes ID cues related to movement
requires attention to safety and disposal
Optional: Live
Samples must be protected from heating
predation occuring in sample alter composition"
Picking Samples: Preserved or live?






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